Yuko Hasegawa1, Yuko Mekata2, Ayaka Sunami3, Yuri Yokoyama3, Takahiro Yoshizaki3, Maya Hagiwara1, Kae Yanagisawa4, Mika Usuda5, Yasunobu Masuda5, Yukari Kawano1

1Tokyo University of Agriculture, Department of Nutritional Science, Tokyo, Japan
2Bunkyo University, Department of Registered Dietitians, Chigasaki, Japan
3Tokyo University of Agriculture, Department of Food and Nutritional Science, Tokyo, Japan
4Sagami Women's University, Department of Nutritional Sciences, Sagamihara, Japan
5Kewpie Corporation, Research & Development Division, Tokyo, Japan

Effect of Egg White Protein Supplementation Prior to Acute Resistance Training on Muscle Damage Indices in Untrained Japanese Men

Monten. J. Sports Sci. Med. 2014, 3(2), 5-12


The purpose of this study was to assess the effects of egg white (E) protein supplementation on the muscle damage indices and muscular soreness after acute resistance training (RT) compared with soy (S) or no protein supplementation (C). In this cross-over study, six healthy untrained men completed three RT trials. Participants were asked to consume a meat-free diet and refrain from high-intensity activities during all trial periods. On the day of RT, participants ingested one of three test beverages containing water only or water containing either 20 g of E or S protein 1.5 hours after breakfast, then performed 60 minutes of RT. Blood was drawn at baseline, before, immediately after, and 30 minutes after RT to assess blood glucose, lactate, insulin, growth hormone (GH), creatine kinase activity (CK) and cortisol levels. Urinary 3-methylhistidine (3-MetHis), urea nitrogen (UN), and creatinine (CRE) were measured using 24-h urine samples, and muscular soreness was measured by a visual analog scale. The daily protein intake was approximately 0.8 g/kg body weight in all three groups. Each lactate, GH, CK, cortisol, 3-MetHis, or muscular soreness increased significantly after RT, with no significant differences between the three groups. The UN was significantly higher in the E and S groups compared to the C group. The RT exercise protocol successfully induced blood biochemical changes, muscle damage or muscle soreness in all three groups with no significant differences, and pre-exercise protein supplementation taken in excess may accelerate protein catabolism.


3-methylhistidine, urea nitrogen, muscle soreness

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Bachle, T.R., Earle, R.W., & Wathen, D. (2000). Resistance training. In Bachle, T.R. & Earle, R.W (Eds.), Essentials of Strength Training and Conditioning (2nd ed.). (pp. 395-425). Champaign, IL: Human Kinetics.

Bird, S.P., Tarpenning, K.M., & Marino, F.E. (2006). Liquid carbohydrate/essential amino acid ingestion during a short-term bout of resistance exercise suppresses myofibrillar protein degradation. Metabolism, 55(5), 570-577. https://doi.org/10.1016/j.metabol.2005.11.011

Børsheim, E., Tipton, K.D., Wolf, S.E., & Wolfe, R.R. (2002). Essential amino acids and muscle protein recovery from resistance exercise. American Journal of Physiological Endocrinology and Metabolism, 283(4), E648-E657. https://doi.org/10.1152/ajpendo.00466.2001

Buckley JD, Thomson RL, Coates AM, Howe PR, DeNichilo MO, & Rowney MK. (2010). Supplementation with a whey protein hydrolysate enhances recovery of muscle force-generating capacity following eccentric exercise. Journal of Science and Medicine in Sport, 13(1), 178-181. https://doi.org/10.1016/ j.jsams.2008.06.007

Candow, D.G., Chilibeck, P.D., Facci, M., Abeysekara, S., & Zello, G.A. (2006). Protein supplementation before and after resistance training in older men. European Journal of Applied Physiology, 97(5), 548-556. https://doi.org/10.1007/s00421-006-0223-8

Candow, D.G., Little, J.P., Chilibeck, P.D., Abeysekara, S., Zello, G.A., Kazachkov, M., Cornish, S.M., & Yu, P.H. (2008). Low-dose creatine combined with protein during resistance training in older men. Medicine and Science in Sports and Exercise, 40(9), 1645-1652. https://doi.org/10.1249/MSS.0b013e31817 6b310

Evans, W.J. (1992). Exercise, nutrition and aging. Journal of Nutrition, 122 (3 suppl), 796-801.

Ghanbari-Niaki, A., Nabatchian, S., & Hedayati, M. (2007). Plasma agouti-related protein (AGRP), growth hormone, insulin responses to a single circuit-resistance exercise in male college students. Peptides, 28(5), 1035-1039. https://doi.org/10.1016/j.peptides.2007.02.004

Gilani, G.S. (2012). Background on international activities on protein quality assessment of foods. British Journal of Nutrition, 108 (2), S168-S182. https://doi.org/10.1017/S000711451 2002383

Green, M.S., Corona, B.T., Doyle, J.A., & Ingalls, C.P. (2008). Carbohydrate-protein drinks do not enhance recovery from exercise-induced muscle injury. International Journal of Sport Nutrition and Exercise Metabolism, 18(1), 1-18.

Hoffman, J.R., Ratamess, N.A., Ross, R., Shanklin, M., Kang, J., & Faigenbaum, A.D. (2008). Effect of a pre-exercise energy supplement on the acute hormonal response to resistance exercise. Journal of Strength and Conditioning Research, 22(3), 874-882. https://doi.org/10.1519/JSC.0b013e31816d 5db6

Howatson, G., Hoad, M., Goodall, S., Tallent, J., Bell, P.G., & French, D.N. (2012). Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. Journal of the International Society of Sports Nutrition, 9(1), 20. Retrieved from http://www.jissn.com/content/9/1/20

Lukaski, H.C., Mendez, J., Buskirk, E.R., & Cohn, S.H.(1981). Relationship between endogenous 3-methylhistidine excretion and body composition. American Journal of Physiology, 240 (3), E302-E307.

Moore, D.R., Robinson, M.J., Fry, J.L., Tang, J.E., Glover, E.I., Wilkinson, S.B., Prior, T., Tarnopolsky, M.A., & Phillips, S.M. (2009). Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. American Journal of Clinical Nutrition, 89 (1), 161-168. https://doi.org/10.3945/ajcn.2008.26401

Nelson, M.R., Conlee, R.K., & Parcell, A.C. (2004). Inadequate carbohydrate intake following prolonged exercise does not increase muscle soreness after 15 min of downhill running. International Journal of Sport Nutrition and Exercise Metabolism, 14(2): 171-184.

Phillips, S.M., Hartman, J.W., & Wilkinson, S.B. (2005). Dietary protein to support anabolism with resistance exercise in young men. Journal of the American College Nutrition, 24(2), 134S-139S.

Roy, B.D., Tarnopolsky, M.A., MacDougall, J.D., Fowles, J., & Yarasheski, K.E. (1997). Effect of glucose supplement timing on protein metabolism after resistance training. Journal of Applied Physiology, 82 (6), 1882-1888.

Roy, B.D., Fowles, J.R., Hill, R., & Tarnopolsky, M.A. (2000). Macronutrient intake and whole body protein metabolism following resistance exercise. Medicine and Science in Sports and Exercise, 32(8), 1412-1418.

Tang, J.E., Moore, D.R., Kujbida, G.W., Tarnopolsky, M.A., & Phillips, S.M. (2009). Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men, Journal of Applied Physiology, 107(3), 987-992. https://doi.org/10.1152/japplphysiol.00076.2009

West, D.W., & Phillips, S.M. (2012). Associations of exercise-induced hormone profiles and gains in strength and hypertrophy in a large cohort after weight training. European Journal of Applied Physiology, 112 (7), 2693-2702. https://doi.org/10.1007/ s00421-011-2246-z

Young, V.R., El-Khoury, A.E., Raguso, C.A., Forslund, A.H., & Hambraeus, L. (2000). Rates of urea production and hydrolysis and leucine oxidation change linearly over widely varying protein intakes in healthy adults. Journal of Nutrition, 130(4), 761-766.