Seyed Morteza Tayebi1, Karsten Krüger2, Farahnaz Ebrahimi3, Abbas Izadi3, Maryam Roushan3, Anna V. Nenasheva4

1Allameh Tabataba’i University, Faculty of Sport Science, Department of Exercise Physiology, Tehran, Iran
2Justus-Liebig-Universität Gießen, Department of Exercise Physiology and Sports Therapy, Gießen, Germany
3Islamic Azad University, Kerman Branch, Faculty of Humanity, Department of Exercise Physiology, Kerman, Iran
4South Ural State University (National Research University), Institute of Sport, Tourism and Service, Athletic Performance Enhancement Department, Chelyabinsk, Russia

Supplementation with Ziziphus Jujuba Suppresses Apoptosis Signals in Neutrophils after Acute Exercise

Monten. J. Sports Sci. Med. 2021, 10(2), 31-39 | DOI: 10.26773/mjssm.210905


It is suggested that jujube might benefit exercise-induced immune fluctuations, specifically on neutrophils’ apoptosis regulation, but its cellular mechanism is unclear. This study aimed to investigate the effect of one-week supplemen- tation with Ziziphus jujuba on pro- and anti-apoptotic protein levels of neutrophils in response to a session of circuit resistance exercise. Fourteen young, healthy male students completed a session of circuit resistance exercise (75% 1RM, nine exercises, three sets) in two groups (n=7). While one group received a placebo, the other group was supplemented with jujube (0.5 g/kg body weight suspended in 2.5 cc distilled water) started a weekday before the exercise session. Blood samples were collected 30 minutes before, immediately, and two hours after the exercise. Neutrophils were col- lected and pro- (Calpain-1, Bax, Caspase-3) and anti-apoptotic (Calpastatin, XIAP) proteins measured with ELISA. In- tracellular calcium ([Ca 2+ ]i) was assessed using the Atomic Absorption/ Flame Emission method. Repeated-Measures ANOVA was used for the interaction effects of TIME×GROUP (3×2) at the significance level (p) of 0.05. The SPSS software was used for analyses. Levels of ([Ca 2+ ]i), expression of calpain-, and caspase-3 were increased in response to circuit resistance exercise (p<0.05). In contrast, supplementation with jujube suppressed these changes (p<0.01). The data indicate that a single session of intensive circuit resistance exercise elevated apoptosis signalling in human neutrophils with the involvement of [Ca 2+ ]i-Calpastatin-Calpain axis upstream caspase-3. Supplementation with the jujube solution attenuated cell death signalling, possibly by providing energy for neutrophils. Otherwise, the improvement of the anti- oxidant status might be protective against ROS-induced apoptosis during exercise.


exercise, resistance exercise, ziziphus jujuba, neutrophil cell, cell apoptosis, intracellular calcium

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