1Yarmouk University, Faculty of Physical Education, Department of Sports/Movement Sciences, Irbid,
Effect of Oral Supplementation with L-Carnitine on Performance Time in a 5000 m Race and Responses of Free Fatty Acid and Carnitine Concentrations in Trained-Endurance Athletes
This study was designed to determine the effect of oral supplementation with L-carnitine on the performance time in a 5000 m race. In addition, free fatty acid, blood carnitine, lactate, and glucose responses to the race following the supplementation period were measured. Twenty male trained-endurance athletes were randomly divided into two groups (L-carnitine, n = 10 (22.13 ± 2.66 yrs) or placebo, n = 10 (21.63 ± 2.23 yrs)). The study was performed with a randomized, double-blind, placebo-controlled parallel-group, in which participants ingested an L-carnitine supplement or a placebo 2 × 1.5 g/day for 3 weeks. Athletes completed a 5000 m race before and after the supplementation period. Blood samples were collected from each athlete before and after the race, preand post-supplementation to measure the physiological responses. Data showed that there were no differences in performance time before (p=0.624) and after (p=0.407) supplementation period between groups and within a group (p>0.05). No differences existed in physiological responses between groups after supplementation before beginning the race (p>0.05), except for the blood carnitine level, which was significantly higher in the L-carnitine than the placebo (P=0.001) group. After the finish of the race, however, data showed better physiological responses in response to L-carnitine supplementation compared to the placebo group (p<0.05). In conclusion, although L-carnitine supplementation increases blood carnitine concentration, it has no beneficial effect on performance time of 5000 m race probably due to the short duration of the race; it might also have no ergogenic effect.
β-oxidation, mitochondria, neuropathies, nitric oxide, running economy
Abbias, C.R., & Laursen, P.B. (2005). Models to explain fatigue during prolonged endurance cycling. Sports Medicine, 35(10), 865-898. https://doi.org/10.2165/00007256-200535100-00004
Bavari, M., Tabandeh, M.R., Varzi, H.N., & Bahramzadeh, S. (2016). Neuroprotective, antiapoptotic and antioxidant effects of L-carnitine against caffeine-induced neurotoxicity in SH-SY5Y neuroblastoma cell line. Drug and Chemical Toxicology, 39(2), 157-166. https://doi.org/10.3109/01480545.2015.1063062
Belcastro, A.N., Albisser, T.A., & Litteljohn, B. (1996). Role of calcium-activated neural protease (calpain) with diet and exercise. Canadian Journal of Applied Physiology, 21, 328-346. https://doi.org/10.1139/h96-029
Bene, J., Csiky, B., Komlosi, K., Sulyok, E., & Melegh, B. (2011). Dynamic adaptive changes of the serum carnitine esters during and after L-carnitine supplementation in patients with maintenance haemodialysis. Scandinavian Journal of Clinical & Laboratory Investigation, 71, 280-286. https://doi.org/10.3109/00365513.2011.560674
Brass, E.P., & Hiatt, W.R. (1998). The role of carnitine and carnitine supplementation during exercise in man and in individuals with special needs. Journal of American College of Nutrition, 17(3), 207-215. https://doi.org/10.1080/07315724.1998.10718750
Broad, E., Bolger, C., & Galloway, S. (2006). Dietary carnitine intake and carnitine status in endurance-trained males. Nutrition & Dietetics, 63, 148-154. https://doi.org/10.1111/j.1747-0080.2006.00068.x
Brouns, F., Saris, W.H., Beckers, E., Aldercreutz, H., van der Vusse, G.J., Keizer, H.A., Kuipers, H., Menheere, P., Wagenmakers, A.J., & ten Hoor, F. (1989). Metabolic changes induced by sustained exhaustive cycling and diet manipulation. International Journal of Sports Medicine, 10 (suppl 1), S49-62. https://doi.org/10.1055/s-2007-1024954
Delaš, I., Dražić, T., Čačić-Hribjan, M., &Sanković, K. (2008). Effect of L-carnitine supplementation on some biochemical parameters in blood serum of sedentary population. Croatica Chemica Acta Ccacaa, 81(1), 163-168.
Demarquory, J., Geofges, B., Rigault, C., Royer, M., Clairet, A., Soty, M., Lekounoungou, S., & Le Borgne, F. (2004). Radioisotopic determination of L-carnitine content in foods commonly eaten in Western countries. Food Chemistry, 86, 137-142. https://doi.org/10.1016/j.foodchem.2003.09.023
Eizadi, M., Pourvaghar, A.K., Nazem, F., Eghdami, A., & Khorshidi, D. (2009). The determination of acute oral L-carnitine ingestion on physiological and biochemical parameters related with lipids in endurance exercise. Journal of Babol University of Medical Sciences, 11(5), 45-51.
Gandevia, S.C. (2001). Spinal and supraspinal factors in human muscle fatigue. Physiological Reviews, 81(4), 1725-1789. https://doi.org/10.1152/physrev.2001.81.4.1725
Greig, C., Finch, K.M., Jones, D.A., Coopr, M., Sargeant, A.J., & Forte, C.A. (1987). The effect of oral supplementation with L-carnitine on maximum and submaximum exercise capacity. European Journal of Applied Physiology, 56, 475-460. https://doi.org/10.1007/BF00417775
Hanon, C., Thépaut-Mathieu, C., & Vandewalle, H. (2005). Determination of muscular fatigue in elite runners. European Journal of Applied Physiology, 94, 118-125. https://doi.org/10.1007/s00421-004-1276-1
Kashef, M., & Saei, M.A. (2017). Acute effect of L-carnitine supplementation on lactate, glucose, saturated oxygen and VO2max variations in young males. International Journal of Basic Science in Medicine, 2(1), 46-51. https://doi.org/10.15171/ijbsm.2017.10
Lennon, D.L.F., Shrago, E.R., Madden, M., Nagle, F.J., & Hanson, P. (1986). dietary carnitine intake related to skeletal muscle and plasma carnitine concentrations in adult men and women. Food Chemistry, 86, 137-142. https://doi.org/ 10.1093/ajcn/43.2.234
Miklos, A., Ciulea, L., Vari, C.E., Imre, S., Ősz, BE.,& Tero-Vescan, A. (2016). The efficiency and safety of L-carnitine and cafeeine after short-and long-term administration. Palestrica of the third millenium-Civlization and Sport, 17(3), 229-232.
Mojtaba, E., Laleh, B., Mohsen, S., & Zohreh, A. (2011). Fat metabolism and aerobic capacity does not affect by acute L-carnitine-L-tartrate supplementation. Journal of Applied Environmental and Biological Sciences, 1(12), 695-699.
Pekala, J., Patkowska-Sokola, B., Bodkoeski, R., Jamroz, D., Nowakowski, P., Lochynski, S., & Librowski, T. (2011). L-Carnitine: metabolic functions and meaning in humans life. Current Drug Metabolism, 12(7), 667-678. https://doi.org/10.2174/138920011796504536
Peters, L.W.E., Smiet, E., de Sain-van der Velden, M.G.M., & van der Kolk, J.H. (2015). Acylcarnitine ester utilization by the hindlimb of warm blood horses at rest and following low intensity exercise and carnitine supplementation. Veterinary Quarterly, 35(2), 76-81. https://doi.org/10.1080/01652176.2015.1027039
Petersen, K., Hansen, C.B., Aagaard, P., & Madsen, K. (2007). Muscle mechanical characteristics in fatigue and recovery from a marathon race in highly trained runners. European Journal of Applied Physiology, 101, 385-396. https://doi.org/10.1007/s00421-007-0504-x
Rebouche, C.J. (1992). Carnitine function and requirements during the life cycle. FASEB, 6, 3379-3386. https://doi.org/ 10.1096/fasebj.6.15.1464372
Siddiqui, M.K., Mughal, S.A., Siddiqui, M.S., & Hayat, A.S. (2015). Effects of L-carnitine; on skeletal muscle of rabbit. Professional Medical Journal, 22 (8), 1001-1005.
Smith, W.A., Fry, A.C., Tschume, L.C., & Bloomer, R.J. (2008). Effect of glycine propionyl-L-carnitine on aerobic and anaerobic exercise performance. International Journal of Sport Nutrition and Exercise Metabolism, 18, 19-36. https://doi.org/10.1123/ijsnem.18.1.19
Stumpf, D.A., Parker, W.D., & Angelini, C. (1985). Carnitine deficiency, organic acidemias, and Reye's syndrome. Neurology, 35(7), 1041-1045.
Tarnopolsky, M. (2004). Protein requirements for endurance athletes. European Journal of Sport Science, 4(1), 1-16. https://doi.org/10.1080/17461390400074102
Vecchiet, L., Di Lisa, F., Pieralisi, G., Ripari, P., Menabo, R., Giamberardino, M.A., & Siliprandi, N. (1990). Influence of L-carnitine administration on maximal physical exercise. European Journal of Applied Physiology, 61, 486-490. https://doi.org/10.1007/BF00236072
Verges, S., Flore, P., Favri-Juvin, A., Lévy, P., & Wuyam, B. (2005). Exhaled nitric oxide during normoxic and hypoxic exercise in endurance athletes. Acta Physiologica, 185, 123-131. https://doi.org/10.1111/j.1365-201X.2005.01475.x
Wächter, S., Vogt, M., & Kreis, R. (2002). Long-term administration of L-carnitine to humans: effect on skeletal muscle carnitine content and physical performance. Clinica Chimica Acta, 318(1), 51-61. https://doi.org/10.1016/S0009-8981(01)00804-X
Wall, B.T., Stphens, F.B., Van Loon, L.J.C., Constantin-Teodosiu, D., Macdonald, I.A., & Greenhaff, P.L. (2013). Reduced fat oxidation during high intensity, submaximal exercise: is the availability of carnitine important? European Journal of Sport Science, 13(2), 191-199. https://doi.org/10.1080/17461391.2011.630103
Zhang, R., Zhang, H., Zhang, Z., Wang, T., Niu, J., Cui, D., & Xu, S. (2012). Neuroprotective effects of pre-treatment with L-carnitine and acetyl-L-carnitine on ischemic injury in vivo and in vitro. International Journal of Molecular Sciences, 13 (2), 2078-2090. https://doi.org/https://doi.org/10.3390/ijms13022078